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10x translation buffer  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher 10x translation buffer
    10x Translation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/translation+buffer/HEPES/pm41465210-306-15-57
    Average 99 stars, based on 1 article reviews
    10x translation buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Affinity Purification:

    Article Title: An Intrinsically Disordered RNA Binding Protein Modulates mRNA Translation and Storage.
    Article Snippet: .. Following heparin affinity purification, His-tagged Sbp1 was exchanged to the translation buffer (22 mM Hepes-KOH, pH 7.4, 120 mM potassium acetate, 2 mM magnesium acetate, 0.75 mM ATP, 0.1 mM GTP, 25 mM creatine phosphate [Invitrogen], 1.67 mM 1,4-dithiothreitol (DTT)) and concentrated to a final concentration of 25μM. ..

    Concentration Assay:

    Article Title: An Intrinsically Disordered RNA Binding Protein Modulates mRNA Translation and Storage.
    Article Snippet: .. Following heparin affinity purification, His-tagged Sbp1 was exchanged to the translation buffer (22 mM Hepes-KOH, pH 7.4, 120 mM potassium acetate, 2 mM magnesium acetate, 0.75 mM ATP, 0.1 mM GTP, 25 mM creatine phosphate [Invitrogen], 1.67 mM 1,4-dithiothreitol (DTT)) and concentrated to a final concentration of 25μM. ..

    Solvent:

    Article Title: Mining Translation Inhibitors by a Unique Peptidyl-Aminonucleoside Synthetase Reveals Cystocin Biosynthesis and Self-Resistance
    Article Snippet: .. The total volume of the translation reaction was 10 μL, including 5 μL HEK293T extract, 1 μL 10× translation buffer (20 mM Hepes-KOH pH 7.6, 1 mM DTT, 0.5 mM spermidine-HCl, 0.8 mM Mg(OAc) 2 , 8 mM creatine phosphate, 1 mM ATP, 0.2 mM GTP, 120 mM KOAc, and 25 μM of each amino acid), 2U of RiboLock RNase inhibitor (Thermo Scientific, USA), 0.5 mM d-luciferin (Promega), 1 μL of either antibiotic solution or solvent (water), as indicated, and 50 ng mRNA (the latter was added in 1 μL water solution after the reaction mixture supplemented with antibiotic was pre-incubated for 5 min at 30 °C). ..

    Article Title: Mining Translation Inhibitors by a Unique Peptidyl-Aminonucleoside Synthetase Reveals Cystocin Biosynthesis and Self-Resistance.
    Article Snippet: .. The total volume of the translation reaction was 10 μL, including 5 μL HEK293T extract, 1 μL 10× translation buffer (20 mM Hepes-KOH pH 7.6, 1 mM DTT, 0.5 mM spermidine-HCl, 0.8 mM Mg(OAc)2, 8 mM creatine phosphate, 1 mM ATP, 0.2 mM GTP, 120 mM KOAc, and 25 μM of each amino acid), 2U of RiboLock RNase inhibitor (Thermo Scientific, USA), 0.5 mM d-luciferin (Promega), 1 μL of either antibiotic solution or solvent (water), as indicated, and 50 ng mRNA (the latter was added in 1 μL water solution after the reaction mixture supplemented with antibiotic was pre-incubated for 5 min at 30 ◦C). ..



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